亚洲一区二区三区看片,日韩欧美九九九久久久,精品国产日韩亚洲一区在线,久久噜噜噜久久熟女精品

掃碼關注公眾號           掃碼咨詢技術支持           掃碼咨詢技術服務
  
客服熱線:400-901-9800  客服QQ:4009019800  技術答疑  技術支持  質量反饋  人才招聘  關于我們  聯(lián)系我們
国产品无码一区二区三区在线,精品久久久777777麻豆
首頁 > 產品中心 > 標記一抗 > 產品信息
Rabbit Anti-phospho-CHK1 (Ser286)/PE-Cy3 Conjugated antibody (bs-13906R-PE-Cy3)
訂購熱線:400-901-9800
訂購郵箱:sales@www.lalhoau.cn
訂購QQ:  400-901-9800
技術支持:techsupport@www.lalhoau.cn
說 明 書: 100ul  
100ul/2980.00元
大包裝/詢價
產品編號 bs-13906R-PE-Cy3
英文名稱1 Rabbit Anti-phospho-CHK1 (Ser286)/PE-Cy3 Conjugated antibody
中文名稱 PE-Cy3標記的磷酸化細胞周期檢測點激酶1抗體
別    名 Chk1 (phospho S286); p-Chk1 (phospho S286); Chk1 (Phospho-Ser286); CHEK1 (phospho Ser286); CHEK1 (phospho Ser286); p-CHEK1 (Ser286); Cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog (S. pombe); CHEK 1; CHEK-1; CHEK1; Chk 1; Chk1; CHK1 checkpoint homolog (S. pombe); EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae); C85740; CHK1_HUMAN.  
規(guī)格價格 100ul/2980元 購買        大包裝/詢價
說 明 書 100ul  
產品類型 磷酸化抗體 
研究領域 細胞生物  染色質和核信號  信號轉導  細胞周期蛋白  激酶和磷酸酶  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 (predicted: Human, Chicken, Dog, Horse, )
產品應用 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 54kDa
性    狀 Lyophilized or Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser286.
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產品介紹 background:
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011].

Function:
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest. Isoform 2: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.

Subunit:
Interacts (phosphorylated by ATR) with RAD51. Interacts with and phosphorylates CLSPN, an adapter protein that regulates the ATR-dependent phosphorylation of CHEK1. Interacts with BRCA1. Interacts with and phosphorylates CDC25A, CDC25B and CDC25C. Interacts with FBXO6, which regulates CHEK1. Interacts with PPM1D, which regulates CHEK1 through dephosphorylation. Interacts with TIMELESS; DNA damage-dependent. Interacts with FEM1B; activates CHEK1 in response to stress. Interacts with TLK1. Interacts with XPO1 and YWHAZ. Isoform 1 associates with isoform 2, the interaction is disrupted upon phosphorylation by ATR.

Subcellular Location:
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Note=Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.

Tissue Specificity:
Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon. Post-translational modifications : Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 induces a change in the conformation of the protein, activates the kinase activity and is a prerequisite for interaction with FBXO6 and subsequent ubiquitination at Lys-436. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. Phosphorylation at Ser-280 by AKT1/PKB, may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, resulting in decreased activity.

Similarity:
Belongs to the protein kinase superfamily.
CAMK Ser/Thr protein kinase family. NIM1 subfamily.
Contains 1 protein kinase domain.

Database links:

Entrez Gene: 1111 Human

Omim: 603078 Human

SwissProt: O14757 Human

Unigene: 24529 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Chk1作為Cdks的調節(jié)參與細胞周期調節(jié)過程,是生物進化過程中非常保守的蛋白激酶,在DNA損傷引起的細胞周期檢測點調節(jié)中有著非常重要的作用.在細胞周期檢測點信號傳導通路中,Chkl和Chk2起著重要作用,主要參與G2/M期細胞周期檢測點信號傳導.
版權所有 2004-2026 www.www.lalhoau.cn 北京博奧森生物技術有限公司
通過國際質量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫(yī)療器械-質量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網安備110107000727號
红安县| 柳州市| 安远县| 太湖县| 平邑县| 德庆县| 库伦旗| 乐陵市| 东海县| 威信县| 赫章县| 凌云县| 新绛县| 高雄县| 莱西市| 开江县| 凌云县| 交城县| 治县。| 平远县| 五大连池市| 宁德市| 剑川县| 林西县| 繁峙县| 玛纳斯县| 泽州县| 巴彦县| 扶余县| 京山县| 义马市| 且末县| 韶山市| 米泉市| 曲阜市| 库尔勒市| 弋阳县| 岳阳市| 泰兴市| 铜山县| 名山县|