| 產(chǎn)品編號(hào) | bs-0300R-FITC |
| 英文名稱1 | Rabbit Anti-Mesothelin/FITC Conjugated antibody |
| 中文名稱 | FITC標(biāo)記的間皮素抗體 |
| 別 名 | Mesothelin; MES; CAK 1; CAK1; CAK1 antigen; Megakaryocyte potentiating factor; Mesothelin isoform 1 precursor; MPF; MSLN; Pre pro megakaryocyte potentiating factor; SMR; SMRP; Soluble MPF mesothelin related protein; MSLN_HUMAN. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說(shuō) 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 腫瘤細(xì)胞生物標(biāo)志物 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 64kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Mesothelin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Mesothelin(MSLN)may play a role in cell adhesion and cell shape dynamics; human homolog highly expressed in renal cell carcinoma. malignant tumors, such as a skin lump, ovary cancer and the pancreas cancer...etc..Mainly used for the research of the malignant tumor. Function: Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Subunit: Interacts with MUC16. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus. Megakaryocyte-potentiating factor: Secreted. Isoform 3: Secreted. Tissue Specificity: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). Post-translational modifications: Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. Similarity: Belongs to the mesothelin family. Database links: Entrez Gene: 10232 Human Omim: 601051 Human SwissProt: Q13421 Human Unigene: 408488 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 間皮素（mesothelin, MSLN）是一種相對(duì)分子量：64kDa的細(xì)胞表面糖蛋白, 在間皮瘤、 卵巢癌、 胰腺癌等惡性腫瘤中有較高的表達(dá)。主要用于惡性腫瘤的研究。 |
| 產(chǎn)品圖片 |
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Mesothelin antibody (bs-0300R-FITC)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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