亚洲一区二区三区看片,日韩欧美九九九久久久,精品国产日韩亚洲一区在线,久久噜噜噜久久熟女精品

掃碼關注公眾號           掃碼咨詢技術支持           掃碼咨詢技術服務
  
客服熱線:400-901-9800  客服QQ:4009019800  技術答疑  技術支持  質量反饋  人才招聘  關于我們  聯系我們
91国产精品福利在线观看,欧美美熟你色a亚洲国产精品,精品久久久bbbb人妻
首頁 > 產品中心 > 常用試劑 > 產品信息
BCA Protein Assay Kit (C05-02001)  
訂購熱線:400-901-9800
訂購郵箱:sales@www.lalhoau.cn
訂購QQ:  400-901-9800
技術支持:techsupport@www.lalhoau.cn
說明書: 下載
40ml(200T)/180.00元
100ml(500T)/318.00元
100ml×10(5000T)/2400.00元
大包裝/詢價

產品編號 C05-02001
英文名稱 BCA Protein Assay Kit
中文名稱 BCA蛋白定量試劑盒
別    名 BCA Kit; bicinchoninic acid; BCA Assay Kit; Bicinchoninic Acid Kit for Protein Determination; BCA Protein Quantitative Kit;   BCA蛋白濃度測定試劑盒; BCA法蛋白質含量測定; BCA蛋白質定量試劑盒; BCA蛋白質含量試劑盒; BCA蛋白質含量檢測試劑盒; BCA蛋白定量試劑盒; BCA蛋白定量試劑盒; BCA試劑盒; 蛋白含量測定; BCA 蛋白定量分析試劑盒;
Specific References  (35)     |     C05-02001 has been referenced in 35 publications.
[IF=13.281] Qin Ouyang. et al. Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease. 2022 Feb 19  Other ;  Other.  
[IF=10.107] Zhao Yin. et al. Discovery of Berberine that Targetedly Induces Autophagic Degradation of both BCR-ABL and BCR-ABL T315I through Recruiting LRSAM1 for Overcoming Imatinib Resistance. Clin Cancer Res. 2020 Aug;26(15):4040-4053  other ;  
[IF=9.417] Wen Zhang. et al. Construction of an ornidazole/bFGF-loaded electrospun composite membrane with a core-shell structure for guided tissue regeneration. MATER DESIGN. 2022 Sep;221:110960  
[IF=8.911] Yin Z et al. Discovery of berberine that targetedly induce autophagic degradation of both BCR-ABL and BCR-ABL T315I through recruiting LRSAM1 for overcoming imatinib-resistance. Clin Cancer Res. 2020 Feb 25.  Other ;  
[IF=7.666] Weiheng Zhang. et al. Long-Term Treatment with Gadopentetic Acid or Gadodiamide Increases TRPC5 Expression and Decreases Adriamycin Nuclear Accumulation in Breast Cancer Cells. CELLS-BASEL. 2023 Jan;12(9):1304  
[IF=7.31] Zitong Ma. et al. Epimedii Folium and Ligustri Lucidi Fructus Promote Osteoblastogenesis and Inhibit Osteoclastogenesis against Osteoporosis via Acting on Osteoblast-Osteoclast Communication. OXID MED CELL LONGEV. 2023 Jan 31;2023:7212642  
[IF=7.035] Jialiang Wei. et al. Protective role of TLR9-induced macrophage/microglia phagocytosis after experimental intracerebral hemorrhage in mice. CNS NEUROSCI THER. 2022 Jul;:  
[IF=6.706] Zefu Chen. et al. Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins. NUTRIENTS. 2022 Jan;14(24):5222  
[IF=6.638] Wang Z et al. Light-activatable?dual?prodrug?polymer?nanoparticle?for?precise?synergistic?chemotherapy?guidedby?drug-mediated?computed?tomography?imaging. Acta Biomater.?2019 May 22.  Other ;  
[IF=6.208] Haibo Yao. et al. Whole-Transcriptome Sequencing of Antler Tissue Reveals That circRNA2829 Regulates Chondrocyte Proliferation and Differentiation via the miR-4286-R+1/FOXO4 Axis. INT J MOL SCI. 2023 Jan;24(8):7204  
[IF=5.738] Gao Xiaoqiang. et al. Regulation of Gamma-Aminobutyric Acid Transaminase Expression and Its Clinical Significance in Hepatocellular Carcinoma. FRONT ONCOL. 2022 Jun;0:2857  
[IF=5.56] Wang Haoxia. et al. Influences of flavonoids from Sedum aizoon L. on biofilm formation of Pseudomonas fragi. APPL MICROBIOL BIOT. 2023 Apr;:1-11  
[IF=5.455] Qin, Gaofeng. et al. Shen-Zhi-Ling oral liquid ameliorates cerebral glucose metabolism disorder in early AD via insulin signal transduction pathway in vivo and in vitro. Chin Med-Uk. 2021 Dec;16(1):1-19  WB ;  Mouse.  
[IF=5.195] Zhangbi Wu. et al. Ginsenoside-Rg1 mitigates cardiac arrest-induced cognitive damage by modulating neuroinflammation and hippocampal plasticity. EUR J PHARMACOL. 2023 Jan;938:175431  
[IF=4.962] Yingying Chen. et al. SUZ12 participates in the proliferation of PNH clones by regulating histone H3K27me3 levels. 2022 Jan 06  Other ;  Other.  
[IF=4.547] Li Jie. et al. S100A9 plays a key role in Clostridium perfringens beta2 toxin-induced inflammatory damage in porcine IPEC-J2 intestinal epithelial cells. BMC GENOMICS. 2023 Dec;24(1):1-14  
[IF=3.974] Wei H et al. Alveolar macrophage reaction to PM2. 5 of hazy day in vitro: Evaluation methods and mitochondrial screening to determine mechanisms of biological effect.Ecotoxicol Environ Saf. 2019 Jun 15;174:566-573.  Other ;  
[IF=3.69] Zitong Ma. et al. Comprehensive evaluation of the combined extracts of Epimedii Folium and Ligustri Lucidi Fructus for PMOP in ovariectomized rats based on MLP-ANN methods. J Ethnopharmacol. 2021 Mar;268:113563  WB ;  
[IF=3.654] Mengying Zhang. et al. Preparation of Black pepper (Piper nigrum L.) essential oil nanoparticles and its antitumor activity on triple negative breast cancer in vitro. J FOOD BIOCHEM. 2022 Sep;:e14406  Other ;  Other.  
[IF=3.575] Li Li. et al. Circ_LPAR3 promotes the progression of oral squamous cell carcinoma (OSCC). Biochem Bioph Res Co. 2021 Dec;:  WB ;  Human.  
[IF=3.53] Adil Hassan et al. Anti-atherosclerotic effects of Lactobacillus plantarum ATCC 14917 in ApoE ?/? mice through modulation of proinflammatory cytokines and oxidative stress. Appl Microbiol Biotechnol. 2020 Jul;104(14):6337-6350.  WB ;  Mouse.  
[IF=3.399] SiZhe Yu. et al. NT157 Inhibits HCC Migration via Downregulating the STAT3/Jab1 Signaling Pathway:. Technol Cancer Res T. 2021;():  WB ;  Human.  
[IF=3.318] Songsong Kang. et al. Tandem mass tag-based proteomics analysis reveals the effects of Guri Gumu-13 pill on drug-induced liver injury. J CHROMATOGR B. 2022 Jun;:123353  
[IF=3.241] Tang X et al. Evaluation of CaD on bone structure and bone metabolic changes in senile osteoporosis rats based on MLP-ANN methods. Food Funct. 2019 Nov 25.  Other ;  
[IF=3.093] Guo Xiao. et al. Effects of Transcutaneous Auricular Vagus Nerve Stimulation on Peripheral and Central Tumor Necrosis Factor Alpha in Rats with Depression-Chronic Somatic Pain Comorbidity. Neural Plast. 2020;2020:8885729  
[IF=3.061] Jie Li. et al. Decreased S100A9 expression alleviates Clostridium perfringens beta2 toxin-induced inflammatory injury in IPEC-J2 cells. PEERJ. 2023 Jan;11:e14722  
[IF=2.959] Peng W et al.Expression of osteoprotegerin and receptor activator for the nuclear factor-κB ligand in XACB/LV-bFGF/MSCs transplantation for repair of rabbit femoral head defect necrosis.(2018) J. Cell. Biochem. Oct 18  WB ;  
[IF=2.532] Jianchao Ren. et al. Ejaculatory Duct Obstruction Affects Seminal Vesicle Contractile Efficacy and Smooth Muscle Ultrastructure in a Rat Model. ANDROLOGIA. 2023;2023:5022466  
[IF=2.089] Zhongxue Ye. et al. DNMT3B attenuated the inhibition of TET3 on epithelial-mesenchymal transition in TGF-β1-induced ovarian cancer by methylating the TET3 promoter. REPROD BIOL. 2022 Dec;22:100701  Other ;  Other.  
[IF=2.031] Yujing Wang. et al. SIRT1 promotes proliferation, migration, and invasion of cervical cancer cell and is upregulated by human papillomavirus 16 E7 oncoprotein. Gynecol Obstet Inves. ;:  WB ;  Human.  
保存條件 Store at -20℃.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 簡介
蛋白質定量是蛋白質研究的基礎工作之一。BCA (bicinchoninic acid)是理想的蛋白質定量方法;是當前比Lowry法更優(yōu)越的專用于檢測總蛋白質含量的產品。該方法以快速靈敏、穩(wěn)定可靠,對不同種類蛋白質檢測的變異系數非常小而倍受專業(yè)人士的青睞。BCA法測定蛋白濃度不受絕大部分樣品中的化學物質的影響。在組織細胞裂解實驗中,常用濃度的去垢劑SDS,Triton X-100,Tween不影響檢測結果,但受螯合劑(EDTA,EGTA)、還原劑(DTT,巰基乙醇)和脂類的影響。實驗中,若發(fā)現樣品稀釋液或裂解液本身背景值較高,可試用Bradford蛋白濃度測定試劑盒。該產品不受此影響,因此與之配合使用,可免除您實驗中的許多煩惱。
基本原理:堿性條件下,蛋白將Cu2+還原為Cu+, Cu+與BCA試劑形成紫顏色的絡合物,測定其在562nm處的吸收值,并與標準曲線對比,即可計算待測蛋白的濃度。
BCA試劑的蛋白質測定范圍是10-2000ug/ml

使用說明:
1. 配制工作液: 根據標準品和樣品數量,按體積比A :B (50:1)配制適量BCA工作液,充分混勻。BCA工作液室溫24小時內穩(wěn)定。
2. 稀釋標準品:取10微升標準品用PBS稀釋至100ul(標準品一般可用PBS稀釋),使終濃度為0.5mg/ml。將標準品按0, 1, 2, 4, 8, 12, 16, 20ul加到96孔板的蛋白標準品孔中,加PBS補足到20ul。
3. 加適當體積樣品到96孔板的樣品孔中,補加PBS到20ul。
4. 各孔加入200ul BCA工作液,37℃放置30分鐘。
5. 冷卻到室溫,用酶標儀562nm測定OD值,根據標準曲線計算出蛋白濃度。

注意事項:
1. 在低溫條件或長期保存出現沉淀時,可攪拌或37℃溫育使溶解, 如發(fā)現細菌污染則應丟棄。
2. 樣品中若含有EDTA、EGTA、DTT、硫酸銨、脂類會影響檢測結果,請試用Bradford蛋白濃度測定試劑盒;高濃度的去垢劑也影響實驗結果,可用TCA沉淀去除干擾物質。
3.要得到更為精確的蛋白濃度結果,每個蛋白梯度和樣品均需做復孔, 每次均應做標準曲線。
4.當試劑A和B混合時可能會有渾濁,但混勻后就會消失,工作液在密閉情況下可保存1周。
5.需準備37℃水浴或溫箱、酶標儀或普通分光光度計,測定波長為540-595nm之間,562nm最佳。酶標儀需與96孔酶標板配套使用。使用分光光度計測定蛋白濃度時,試劑盒測定的樣品數量會因此而減 少。使用溫箱孵育時,應注意防止因水粉蒸發(fā)影響檢測結果。


本產品自2018年4月1日起配方升級,規(guī)格和包裝有所調整,請以官網信息為準。
產品圖片
C05-02001
版權所有 2004-2026 www.www.lalhoau.cn 北京博奧森生物技術有限公司
通過國際質量管理體系ISO 9001:2015 GB/T 19001-2016    證書編號: 00124Q34771R2M/1100
通過國際醫(yī)療器械-質量管理體系ISO 13485:2016 GB/T 42061-2022    證書編號: CQC24QY10047R0M/1100
京ICP備05066980號-1         京公網安備110107000727號
广水市| 乐平市| 白玉县| 屏南县| 内乡县| 如皋市| 仙游县| 德江县| 岳阳县| 扶余县| 泾川县| 富顺县| 农安县| 蒙自县| 崇左市| 鄄城县| 河间市| 云霄县| 彩票| 松阳县| 闸北区| 崇州市| 巴林左旗| 会同县| 乌兰浩特市| 垣曲县| 开封县| 遂平县| 剑川县| 灵寿县| 黔西县| 澄江县| 红安县| 海门市| 枣强县| 吴川市| 阿城市| 浦东新区| 浦县| 泸溪县| 万州区|