| 產(chǎn)品編號 | D-9604 |
| 英文名稱 | Cy5,SE |
| 中文名稱 | 熒光標(biāo)記染料Cy5 |
| 別 名 | 熒光染料; 熒光素Cy5 |
| 保存條件 | Store at -20 °C for one year. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC). Due to inherently high extinction coefficient, this dye is also easily detected by naked eye on gels, and in solution. Cy5 become very popular to replace far red fluorescent dyes, because of its high extinction coefficient (as small as 1 nanomol can be detected in gel electrophoresis by naked eye) and of its fluorophore emission maximum in red region, where many CCD detectors have maximum sensitivity, and biological objects have low background. The scanners actually use different laser emission wavelengths (typically 532 nm and 635 nm) and filter wavelengths (550-600 nm and 655-695 nm) to avoid background contamination. They are thus able to easily distinguish colors from Cy3 and from Cy5, and also able to quantify the amount of Cy3 and Cy5 labeling in one sample (multiparametric detection). 菁染料是性能優(yōu)良的熒光標(biāo)記染料，摩爾吸光系數(shù)在熒光染料中是最高的，其琥珀酰亞胺酯是最常用的脂肪氨基標(biāo)記試劑，廣泛用于蛋白、抗體、核酸及其他生物分子的標(biāo)記和檢測。通過改變次甲基鏈的長度, 可改變其熒光發(fā)射波長，每增加一個雙鍵，按照Huoffman 規(guī)則正好紅移約100nm。 菁染料Cy3 和Cy5 已成為基因芯片的首選熒光標(biāo)記物; 另外,Cy5, Cy5.5 和Cy7 的吸收在近紅外區(qū)背景非常低，是熒光強度最高、最穩(wěn)定的長波長染料。特別適合于活體小動物體內(nèi)成像代替放射性元素。 花菁染料Cy5, SE是一種為數(shù)不多的近紅外熒光標(biāo)記染料，它產(chǎn)生的紅色熒光背景低、熒光強度高，而且熒光穩(wěn)定性好。Cy5, SE常被應(yīng)用于生物分子標(biāo)記，熒光成像及其他熒光生物分析，例如標(biāo)記多肽、蛋白質(zhì)和核酸等。Cy5, SE 熒光標(biāo)記染料與蛋白質(zhì)結(jié)合后可以顯著增強熒光強度。 |
