| 產品編號 | bs-19136R |
| 英文名稱 | Rabbit Anti-ZFPL1 antibody |
| 中文名稱 | 鋅指蛋白樣1抗體 |
| 別 名 | D11S750; MCG4; MGC 4; ZFPL 1; Zinc finger protein like 1; Zinc finger protein MCG4; zinc-finger protein in MEN1 region. |
| 研究領域 | 細胞生物 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZFPL1: 31-130/310 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Zinc finger protein-like 1 is a 310 amino acid single-pass membrane protein with two zinc fingers at the N-terminus, the second of which is likely a RING domain. The RING domain, which is a 40-60 amino acid, cysteine-rich domain that binds two atoms of zinc, plays a key role in the ubiquitination pathway. The presence of zinc finger-like and leucine zipper-like domains in ZFPL1 suggests a role in DNA binding and transcriptional regulation. ZFP1 is widely expressed in the golgi apparatus and is involved in maintaining golgi structure and regulating the rate of cargo transport. Function: Required for cis-Golgi integrity and efficient ER to Golgi transport. Involved in the maintenance of the integrity of the cis-Golgi, possibly via its interaction with GOLGA2/GM130. Subcellular Location: Golgi Apparatus and Nuclear; Single-pass membrane protein. SWISS: O95159 Gene ID: 7542 Database links: Entrez Gene: 7542 Human Entrez Gene: 81909 Mouse SwissProt: O95159 Human SwissProt: Q9DB43 Mouse Unigene: 98170 Human Unigene: 289471 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFPL1) Polyclonal Antibody, Unconjugated (bs-19136R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFPL1) Polyclonal Antibody, Unconjugated (bs-19136R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFPL1) Polyclonal Antibody, Unconjugated (bs-19136R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFPL1) Polyclonal Antibody, Unconjugated (bs-19136R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
