| 產(chǎn)品編號 | bs-16126R |
| 英文名稱 | Rabbit Anti-FLJ36180 antibody |
| 中文名稱 | RNF209/環(huán)指蛋白209抗體 |
| 別 名 | EC 6.3.2.-; FLJ36180; MGC138638; MGC138639; Probable E3 ubiquitin-protein ligase TRIML1; RING finger protein 209; RNF209; TRIML_HUMAN; Triml1; Tripartite motif family like protein 1; Tripartite motif family-like protein 1. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) 環(huán)指蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53kDa |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FLJ36180: /351-450/468 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation.TRIML1 (tripartite motif family-like 1), also known as RNF209 (RING finger protein 209), is a 468 amino acid protein that contains one SPRY domain and one RING-type zinc finger. Due to the presence of a RING-type zinc finger motif, TRIML1 may be involved in protein degradation events within the cell. Function: Probable E3 ubiquitin-protein ligase which plays an important role in blastocyst development. Subunit: Interacts with USP5 (By similarity). Similarity: Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. SWISS: Q8N9V2 Gene ID: 339976 Database links: Entrez Gene: 339976 Human Entrez Gene: 244448 Mouse SwissProt: Q8N9V2 Human SwissProt: Q8BVP1 Mouse Unigene: 348618 Human Unigene: 64542 Mouse |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with FLJ36180 polyclonal antibody, unconjugated (bs-16126R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
