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Rabbit Anti-DSPP  antibody (bs-10316R)  
~~~促銷代碼KT202411~~~
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產(chǎn)品編號 bs-10316R
英文名稱 Rabbit Anti-DSPP  antibody
中文名稱 牙本質(zhì)磷蛋白/牙本質(zhì)骨唾液酸糖蛋白/牙本質(zhì)骨唾液酸磷蛋白抗體
別    名 Dentin phosphophoryn; Dentin phosphoprotein; dentin phosphoryn; Dentin sialophosphoprotein; Dentin sialophosphoprotein precursor; Dentin sialoprotein; dentinogenesis imperfecta 1; DFNA39; DGI1; DMP3; DPP; DSP; DSPP_HUMAN; DTDP2.  
Specific References  (13)     |     bs-10316R has been referenced in 13 publications.
[IF=8.079] Luo Bin. et al. Residual periodontal ligament in the extraction socket promotes the dentin regeneration potential of DPSCs in the rabbit jaw. STEM CELL RES THER. 2023 Dec;14(1):1-14  IHC ;  Rabbit.  
[IF=6.684] Ning Wang. et al. miR-6807-5p Inhibited the Odontogenic Differentiation of Human Dental Pulp Stem Cells Through Directly Targeting METTL7A. Front Cell Dev Biol. 2021; 9: 759192  IHC ;  Mouse.  
[IF=6.344] Zheng, Han. et al. miR-140-3p enhanced the osteo/odontogenic differentiation of DPSCs via inhibiting KMT5B under hypoxia condition. Int J Oral Sci. 2021 Dec;13(1):1-10  WB ;  Human.  
[IF=6.022] Huina Liu. et al. LncRNA, PLXDC2‐OT promoted the osteogenesis potentials of MSCs by inhibiting the deacetylation function of RBM6/SIRT7 complex and OSX specific isoform. 2021 Mar 08  IHC ;  Human.  
[IF=5.546] Yang H et al. Homeobox C8 inhibited the osteo‐/dentinogenic differentiation and migration ability of stem cells of the apical papilla via activating KDM1A. J Cell Physiol . 2020 Apr 4.  IHC ;  human.  
[IF=4.963] Shi R et al. Analysis of the characteristics and expression profiles of coding and noncoding RNAs of human dental pulp stem cells in hypoxic conditions.Stem Cell Res Ther. 2019 Mar 12;10(1):89.  WB ;  Human.  
[IF=4.556] Mohammed Zayed. et al. Effects of p-Cresol on Senescence, Survival, Inflammation, and Odontoblast Differentiation in Canine Dental Pulp Stem Cells. Int J Mol Sci. 2020 Jan;21(18):6931  WB ;  Dog.  
[IF=3.869] Zhang Chen. et al. The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla. Stem Cells Int. 2020;2020:8881021  WB ;  Human.  
[IF=3.144] Kü?üKKAYA EREN Selen. et al. Combined effects of bone morphogenetic protein-7 and mineral trioxide aggregate on the proliferation, migration, and differentiation of human dental pulp stem cells. J APPL ORAL SCI. 2022 Sep;30:  ICC ;  Human.  
[IF=3.08] Zhan, Fu‐Liang, Xin‐Yang Liu, and Xing‐Bo Wang. "The Role of MicroRNA‐143‐5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway." Journal of Cellular Biochemistry (2017).  WB ;  Human.  
[IF=2.959] Zhan et al. The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway. (2018) J.Cell.Biochem. 119:536-546  WB ;  Human.  
[IF=2.784] Chen X et al. Modulation of proliferation and differentiation of gingiva?derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair. Send to Int J Mol Med. 2019 Apr 24.  WB ;  Human.  
[IF=2.613] Chao Wang. et al. SFRP2 enhances dental pulp stem cell‐mediated dentin regeneration in rabbit jaw. 2020 Nov 08  IHC ;  Human.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  細(xì)胞周期蛋白  結(jié)合蛋白  細(xì)胞分化  細(xì)胞骨架  細(xì)胞外基質(zhì)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
細(xì)胞定位 細(xì)胞外基質(zhì) 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Dentin sialoprotein: 31-130/1301 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.

Subunit:
Interacts with FBLN7.

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Tissue Specificity:
Expressed in teeth. DPP is synthesized by odontoblast and transiently expressed by pre-ameloblasts.

Post-translational modifications:
DSP is glycosylated.

DISEASE:
Defects in DSPP are the cause of deafness autosomal dominant type 39 with dentinogenesis imperfecta 1 (DFNA39/DGI1) [MIM:605594]. Affected individuals present DGI1 associated with early onset progressive sensorineural high-frequency hearing loss.
Defects in DSPP are the cause of dentinogenesis imperfecta type 1 (DGI1) [MIM:125490]; also known as dentinogenesis imperfecta Shields type 2 (DGI2). DGI1 is an autosomal dominant disorder in which both the primary and the permanent teeth are affected. It occurs with an incidence of 1:8000 live births. The teeth are amber and opalescent, the pulp chamber being obliterated by abnormal dentin. The enamel, although unaffected, tends to fracture, which makes dentin undergo rapid attrition, leading to shortening of the teeth.
Defects in DSPP are a cause of dentinogenesis imperfecta Shields type 3 (DGI3) [MIM:125500]. Patients with DGI3 do not have stigmata of osteogenesis imperfecta. The finding that a single defects in the DSPP gene causes both phenotypic patterns of DGI2 and DGI3 strongly supports the conclusion that these two disorders are not separate diseases but rather the phenotypic variation of a single genetic defect.
Defects in DSPP are the cause of dentin dysplasia type 2 (DTDP2) [MIM:125420]; also known as dentin dysplasia Shields type 2. DTDP2 is an autosomal dominant disorder in which mineralization of the dentine of the primary teeth is abnormal. On the basis of the phenotypic overlap between, and shared chromosomal location with DGI2 it has been proposed that DTDP2 and DGI2 are allelic. From the results of recent studies, it is clear that different types of mutations in DSPP lead to the two different phenotypes.

SWISS:
Q9NZW4

Gene ID:
1834

Database links:

Entrez Gene: 1834 Human

Omim: 124585 Human

SwissProt: Q9NZW4 Human

Unigene: 678914 Human



產(chǎn)品圖片
Sample: EC-9706(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-DSPP (bs-10316R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47/129 kD Observed band size: 129 kD
Sample: LOVO(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti-DSPP (bs-10316R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:140 kD Observed band size: 130 kD
Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: SW480 (Human) Cell Lysate at 30 ug Lane 3: Du145 (Human) Cell Lysate at 30 ug Primary: Anti-DSPP (bs-10316R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 140 kD Observed band size: 130 kD
Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated(bs-10316R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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