| 產(chǎn)品編號 | bs-13507R |
| 英文名稱 | Rabbit Anti-GPR84 antibody |
| 中文名稱 | G蛋白偶聯(lián)受體84抗體 |
| 別 名 | G protein coupled receptor 84; G-protein coupled receptor 84; G protein coupled receptor EX33; GPCR 4; GPCR4; GPR 84; GPR84_HUMAN; GPR84 (EX33); EX33; Inflammation related G protein coupled receptor EX33; Inflammation-related G-protein coupled receptor EX33; GPCR EX33; EX 33; GPCR GPR84; |
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Specific References (2) | bs-13507R has been referenced in 2 publications.
[IF=5.893] Yongxiang Li. et al. Food reward depends on TLR4 activation in dopaminergic neurons. Pharmacol Res. 2021 Jul;169:105659 WB ; Mouse.
[IF=5.561] Weijie Zhao. et al. Mannan Oligosaccharides Promoted Skeletal Muscle Hypertrophy through the Gut Microbiome and Microbial Metabolites in Mice. FOODS. 2023 Jan;12(2):357 WB ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Sheep,Cow,Dog,Horse,Monkey) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPCR EX33/GPR84: 1-100/396 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates, and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. G protein receptor 84 (GPR84), a member of the GCPR 1 family, is an orphan GCPR expressed in bone marrow, brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta, intestine, lung and peripheral blood leukocytes. In activated T cells, GPR84 regulates early interleukin-4 (IL-4) gene expression. Function: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system. Subcellular Location: Cell membrane. Tissue Specificity: Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9NQS5 Gene ID: 53831 Database links: Entrez Gene: 53831 Human Omim: 606383 Human SwissProt: Q9NQS5 Human Unigene: 306199 Human |
| 產(chǎn)品圖片 |
Sample:
SP2/0 (mouse)Cell Lysate at 40 ug
Lung(mouse) Lysate at 40 ug
HL-60 (human)Cell Lysate at 40 ug
Muscle (mouse) Lysate at 40 ug
Primary: Anti- GPR84 (bs-13507R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44kD
Observed band size: 44 kD
Sample: bone (Rat) Lysate at 40 ug
Primary: Anti- GPR84 (bs-13507R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44kD
Observed band size: 44 kD
Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Bone (Rat) Lysate at 40 ug
Lane 4: Lung (Rat) Lysate at 40 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Lane 6: Cerebellum (Rat) Lysate at 40 ug
Lane 7: U937 (Human) Cell Lysate at 30 ug
Lane 8: U87MG (Human) Cell Lysate at 30 ug
Lane 9: THP-1 (Human) Cell Lysate at 30 ug
Primary: Anti-GPR84 (bs-13507R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
