| 產品編號 | bs-2365R |
| 英文名稱 | Rabbit Anti-Eppin antibody |
| 中文名稱 | 附睪蛋白酶抑制蛋白 |
| 別 名 | SPINLW1; WAP7; WFDC7; Variant; Cancer/testis antigen 71; CT71; Epididymal protease inhibitor; Protease inhibitor WAP7; Serine protease inhibitor-like with Kunitz and WAP domains 1; EPPI_MOUSE; WAPfour-disulfide core domain protein 7. |
| 研究領域 | 腫瘤 細胞生物 免疫學 發(fā)育生物學 生長因子和激素 轉錄調節(jié)因子 泛素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Mouse,Rat) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 13kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Eppin: 23-134/134 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. [provided by RefSeq]. Function: Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility (By similarity). Subunit: Monomer (By similarity). Homodimer (By similarity). Homomultimers (By similarity). Interacts (via C-terminus) with SEMG1 (via 164-283 AA) (By similarity). Interacts with LTF (By similarity). Found in a complex with LTF, CLU, EPPIN and SEMG1 (By similarity). Subcellular Location: Secreted. Cell surface. Note=Present on the surface of spermatozoa both before and after capacitation. Tissue Specificity: Expressed in differentiated spermatogonia in testis. Expressed in spermatogonia cell lines GC-1 spg and GC-2spd(ts) as well as in the Leydig tumor cell line MLTC-1 (at protein level). Expressed specifically in epididymis and testis. Expressed predominantly on the postacrosomal region of mouse spermatozoa, in Sertoli cells, Leydig cells, and round spermatids in the testis, and in the principal cells of the cauda epididymidis epithelium. Similarity: Contains 1 BPTI/Kunitz inhibitor domain. Contains 1 WAP domain. SWISS: Q9DA01 Gene ID: 75526 Database links: Entrez Gene: 57119 Human Entrez Gene: 75526 Mouse Omim: 609031 Human SwissProt: O95925 Human SwissProt: Q9DA01 Mouse Unigene: 121084 Human 附睪蛋白酶抑制蛋白(Eppin)是一種睪丸/附睪特異性蛋白,可能與精子在附睪的成熟密切相關. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
