產(chǎn)品編號 | bs-2221R |
英文名稱 | Rabbit Anti-ATEXPA10 antibody |
中文名稱 | 植物延長蛋白(擬南芥) |
別 名 | ARABIDOPSIS THALIANA EXPANSIN A 10; Alpha-expansin 10; At-EXP10; AtEx10; Ath-ExpAlpha-1.1. |
Specific References (1) | bs-2221R has been referenced in 1 publications.
[IF=3.6] Park, Jiyoung, and B. Kang. "AtPGL3 is an Arabidopsis BURP domain protein that is localized to the cell wall and promotes cell enlargement." Frontiers in Plant Science 6 (2015): 412. WB ;
|
|
產(chǎn)品類型 | 植物抗體 |
研究領域 | 植物 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Arabidopsis Thaliana) |
產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 28kDa |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from Arabidopsis Thaliana ATEXPA10: 201-259/259 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
structural constituent of cell wall;
INVOLVED IN: syncytium formation, plant-type cell wall modification during multidimensional cell growth,unidimensional cell growth, plant-type cell wall loosening; LOCATED IN: plant-type cell wall; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Barwin-related endoglucanase.
SWISS: Q9LDR9 Gene ID: 839218 延長蛋白 extensin是存在于植物細胞壁中的羥脯氨酸(Hyp)占有高比例的糖蛋白。細胞壁中含達2—10%,Hyp占構成蛋白質(zhì)的氨基酸的20%以上,在其他蛋白質(zhì)中則絲氨酸(Ser)的含量高。由于可以控制植物細胞的伸展(ex-tersion),故稱為伸展蛋白。在生長旺盛的幼齡期的細胞中含量少,生長速度遲緩時含量增加,因而認為具有對細胞壁不易伸展的作用。其蛋白質(zhì)部分具有-ser-Hyp-Hyp-Hyp-Hyp的氨基酸序列,Hyp殘基許多有3—4個阿拉伯糖所構成的寡糖;而Ser殘基許多具有1—2個半乳糖。脯氨酸羥化是在肽合成后進行,催化此過程的肽基脯氨酸羥化酶需要二價鐵離子,可被鐵離子絡合劑的a′-dipyridyl 所抑制。肽的脯氨酸羥化后,細胞器的糖鏈結合,被分泌到細胞外,納入細胞壁中。 |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |