產(chǎn)品編號 | bs-2683R |
英文名稱 | Rabbit Anti-CLEC2D antibody |
中文名稱 | CLEC2D蛋白抗體 |
別 名 | C-type lectin domain family 2 member D; C type lectin domain family 2 member D isoform 5; CLAX; LLT1; OCIL; Lectin like NK cell receptor; Lectin like transcript 1; Osteoclast inhibitory lectin; CLC2D_HUMAN; CLEC2D; CLEC2D protein; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT-1; Osteoclast inhibitory lectin. |
研究領(lǐng)域 | 免疫學 細胞表面分子 t-淋巴細胞 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Dog,Horse) |
產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg /test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 10kDa |
細胞定位 | 細胞膜 細胞外基質(zhì) |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CLEC2D: 101-207/207 <Extracellular> |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified, but the full-length nature of every transcript has not been defined. Function: Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans. Subunit: Homodimer or heterodimer; disulfide-linked. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Cell surface. Isoform 2, 4: Endoplasmic reticulum. Tissue Specificity: Detected in fetal heart, brain, lung, chondrocytes, perichondrium and osteoblasts, and in adult splenocytes, thymocytes, lymph-node cells, osteoblasts, growth plate chondrocytes and skeltal muscle overlying the bone (at protein level). Ubiquitous. Detected in thymus, bone marrow, lung, gut, heart, skeletal muscle, ovary, spleen, ileum, liver and kidney. Similarity: Contains 1 C-type lectin domain. SWISS: Q9UHP7 Gene ID: 29121 Database links: Entrez Gene: 29121 Human Entrez Gene: 93694 Mouse Omim: 605659 Human SwissProt: Q9UHP7 Human SwissProt: Q91V08 Mouse Unigene: 268326 Human Unigene: 197536 Mouse Unigene: 22573 Rat |
產(chǎn)品圖片 |
Blank control: mouse thymouses(blue)
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).
Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG;
Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody catalog number: bs-2683R;
Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |